How To Calibrate Your Autoclave (Includes 11 Step Sample Procedure) Written by: Scott Mechler “Calibration” is a word that is frequently used in the steam sterilization industry. This blog post will explore what it is, what is involved in doing it right, alternatives, and the potential effects on an existing sterilization process. What is Calibration? At its very basic premise, calibration is bringing the response of a sensor (e.g. […] Tweet Like Share
Biosafety Levels 1, 2, 3 & 4: What’s the Difference? Written by: Arthur Trapotsis Updated 11/3/22: Information about the biosafety level requirements for handling SAR-CoV-2 (COVID-19 coronavirus) can be found here. Safety is of the utmost importance in any laboratory setting, especially when working with autoclaving equipment. In order to ensure the safety of lab personnel, the environment and surrounding communities, the Centers for Disease Control and Prevention (CDC) […] Tweet Like Share
Why To Autoclave Liquids With a Load Probe Written by: Amit Gupta In our previous Sterilization Cycles post on the F0 Cycle, we briefly reviewed the process for autoclaving liquids with a load probe. This post further discusses the benefits of using a load probe for sterilization validation. A load probe is a temperature sensing probe located inside the laboratory’s autoclave chamber. It is configured such that […] Tweet Like Share
Bowie-Dick & Vacuum Leak Tests: Autoclave Sterilization Cycles Written by: Arthur Trapotsis The entire purpose of a laboratory or medical autoclave is to, quite simply, sterilize; however, without the proper conditions for sterility, an autoclave may fall short of its mission. Validation is an essential quality assurance measure designed to ensure that your autoclave operates exactly as intended. There are a number of different ways to validate […] Tweet Like Share
Steam Sterilization Cycles, Part 7: Product Lifecycle Testing in an Autoclave Written by: Arthur Trapotsis In our 7th installment of the Advanced Cycles Series, we explore automated product lifecycle testing in an autoclave. Repeated exposure to an autoclave chamber’s extreme temperature and pressure conditions allows Quality Control Specialists to sharply accelerate product aging and evaluate the lifecycle of a product. Typically, this process requires that an individual (e.g. technician, scientist, engineer, etc) spend countless hours in front of an autoclave. What if you could program the autoclave to run multiple sterilization cycles (over multiple hours or days) with the press of a single button? Read more → Tweet Like Share
Autoclave Steam Sterilization Cycles, Part 6: F0 Cycle Written by: Arthur Trapotsis Autoclave efficacy is highly dependent on time, temperature, and pressure, and these parameters can be manipulated and optimized to create specific sterilization cycles for each application. To help your facility get the most out of your autoclave, we introduce the sixth article in our Steam Sterilization Cycles series: The F0 Cycle (pronounced f-sub-zero or f-sub-oh). Read more → Tweet Like Share
Properly Sizing an Autoclave Chamber for Your Animal Lab Written by: Mike Peters Steam sterilization is routinely used in animal laboratories for two reasons: 1) to sterilize animal cages in order to create a pathogen-free housing environment and 2) to properly dispose of harmful waste products. Since autoclaves are essential for everyday laboratory operation, it is important to select a chamber size with enough capacity to meet the […] Tweet Like Share
University Sustainability Projects: 3 Innovative Schools Making a Creative Difference Written by: Scott Mechler Today, sustainability initiatives are being adopted by nearly every business, organization, and project in the United States—and for good reason. “Going green” not only helps reduce waste and conserve environmental resources, it enables organizations to promote a healthier workplace and save money. Read more → Tweet Like Share
Steam Sterilization Cycles, Part 5: Low Temperature Cycle Written by: Arthur Trapotsis Steam sterilizers play a pivotal role in your lab’s research capabilities and throughput. However, how can you safely and properly sterilize load types that are more temperature-sensitive than others? To help you avoid overheating a specific load type, thereby compromising it, we introduce the fifth article in CSS’ ongoing Steam Sterilization Cycles series: Read more → Tweet Like Share
Common Steam Autoclave Maintenance Questions, Answered Written by: Amit Gupta Keeping your steam autoclaves in proper working order improves productivity, reduces downtime, extends the life of the autoclave, and helps to ensure overall safety in the workplace. To help you with autoclave upkeep, we wrote about the importance of maintenance and shared some basic tips to maintain your equipment in a prior post. In this post, we take the conversation a little deeper, answering common sterilizer maintenance questions and identifying the critical components on an autoclave that should be inspected periodically by a trained service provider. This is not an exhaustive list of all the autoclave components that should be inspected but merely the major ones. Read more → Tweet Like Share
12.10.25 Introducing SterilSteam Sampler, The Steam Condensate Sampler for AAMI ST108 Compliance → Released in 2023, AAMI/ANSI ST108 establishes water quality requirements for water used in the cleaning, rinsing, disinfection, and sterilization of reusable medical devices. In addition to these requirements, ST108 mandates quarterly testing of steam condensate quality to ensure incoming steam does not stain instruments or pose a risk to patient safety. To help healthcare facilities […]
11.6.25 AAMI ST108 & Critical Water for Autoclaves: What ASCs Need to Know → A major step forward in patient safety, ANSI/AAMI ST108 establishes new minimum water quality requirements for water used in the cleaning, rinsing, disinfection, and sterilization of reusable medical devices. Despite providing guidance on water quality management for sterile processing, ST108 has also created confusion, especially regarding test protocols, water treatment, and critical water requirements. In […]
10.20.25 What Is an Autoclave Machine Really Used for? [Applications, Costs & More] → Autoclave machines are mission-critical equipment in a variety of industries, from healthcare to biotech, ensuring equipment and materials are sterilized and safe for use. Whether you’re considering purchasing an autoclave for a new facility, replacing a broken one, or expanding your current sterilization capacity, understanding the ins and outs of autoclave technology is key to […]
