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Frequently Asked Questions

How does autoclaving kill bacteria?

Autoclaves use steam heat to raise temperatures to such a degree that proteins within the cell walls of a microbe break down and begin to coagulate, thereby killing the bacterium and achieving sterilization.

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How does an autoclave work?

Autoclaves use extreme heat in the form of pressurized steam in order to sterilize goods. Similar to a pressure cooker, an autoclave uses a locked door to create a sealed chamber. The air within that chamber is then replaced by steam, which is pressurized until the goods within the chamber have been sufficiently sterilized.

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Does CSS offer a warranty for laboratory autoclaves?

Yes. All of our laboratory autoclaves include a one (1) year warranty covering defective parts and labor, as well as a substantial warranty on the sterilizer chamber. We can also customize your warranty to meet your specific needs. For more information about our product warranty, please contact our service team.

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Does CSS offer eco-friendly options?

Yes. Here at CSS, we’re dedicated to helping our clients protect the environment (while lowering their operating costs) by reducing their water and energy consumption. To that end, our EcoJacket and WaterEco® water-saving systems come standard with all of our laboratory autoclaves, and we offer a range of additional sustainability features.

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Does CSS offer laboratory autoclaves with different chamber volumes?

Yes. We offer a wide selection of autoclave models with chamber volumes ranging from 3.9 cu. ft. (109 L) to 36 cu. ft. (1,019 L). You can view all of our laboratory autoclaves and corresponding chamber dimension and volume information in our laboratory and research autoclave brochure.

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What is the difference between a Class B and a Class N autoclave?

The primary difference between Class B and Class N autoclaves is how they remove air from the chamber prior to sterilization. Class B autoclaves utilize a vacuum pump to completely remove air from the chamber, which enables steam to better penetrate the load. Class N autoclaves use steam from a boiler or generator to create downward displacement, which pushes air out of the chamber.

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What materials can I sterilize using a laboratory autoclave?

Depending on the particular unit, laboratory autoclaves can sterilize a wide variety of goods, including (but not limited to) both solid and liquid media, metal instruments, glass containers, animal cage bedding, biohazardous materials, soil samples and red bag waste.

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What is a laboratory autoclave?

A laboratory autoclave — also known as a steam sterilizer — is a sophisticated piece of equipment designed to use steam heat to kill any microbial life that may be present on a contaminated load.

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I would like to return some parts I am not using. Can I do that?

You may return the parts within 90 days of purchase, but a 35% restocking fee will apply. No unused electronic parts can be returned. Please call CSS to get a RMA number PRIOR to return.

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Can I customize or add other parts to the SterilCARE PAX so I can get the discount?

When a SterilCARE PAX is requested, initially the factory recommendation will be provided. Our parts PAX can be customized to best meet the customer’s needs, but the basic structure of the PAX must stay intact.

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CSS News & Events

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12.10.25

AAMI ST108 Compliance

Introducing SterilSteam Sampler, The Steam Condensate Sampler for AAMI ST108 Compliance →

Released in 2023, AAMI/ANSI ST108 establishes water quality requirements for water used in the cleaning, rinsing, disinfection, and sterilization of reusable medical devices. In addition to these requirements, ST108 mandates quarterly testing of steam condensate quality to ensure incoming steam does not stain instruments or pose a risk to patient safety. To help healthcare facilities […]

11.6.25

Critical Water for Autoclaves

AAMI ST108 & Critical Water for Autoclaves: What ASCs Need to Know →

A major step forward in patient safety, ANSI/AAMI ST108 establishes new minimum water quality requirements for water used in the cleaning, rinsing, disinfection, and sterilization of reusable medical devices. Despite providing guidance on water quality management for sterile processing, ST108 has also created confusion, especially regarding test protocols, water treatment, and critical water requirements. In […]

10.20.25

woman with long dark hair wearing a white lab coat and blue rubber gloves removes trays from an autoclave

What Is an Autoclave Machine Really Used for? [Applications, Costs & More] →

Autoclave machines are mission-critical equipment in a variety of industries, from healthcare to biotech, ensuring equipment and materials are sterilized and safe for use. Whether you’re considering purchasing an autoclave for a new facility, replacing a broken one, or expanding your current sterilization capacity, understanding the ins and outs of autoclave technology is key to […]
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